Rapid purification of synthetic bombesin by countercurrent chromatography on the multi-layer coil planet centrifuge
作者： Martha Knight , Yoichiro Ito , Paul Peters , Carlo Dibello
DOI: 10.1080/01483918508074131
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摘要: Abstract A temperature-controlled multi-layer coil planet centrifuge rapidly yields highly efficient preparative separations of polar compounds. Capability the method was demonstrated on a one-step purification crude synthetic bombesin with two-phase solvent system composed n-butanol/dichloroacetic acid/water (100:1:100). Under an elevated temperature at 45°C to 50°C, peak eluted within two hours. Reversed phase HPLC analysis fractions showed over 98% purity. The may be applicable many other peptides and
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