Method of detecting the presence of live organisms in substances

摘要: The substance to be tested is premixed by gentle agitation in a glass cuvette with an excess of firefly essence and buffer solution cutting agent, so that any live organisms present are still alive intact after the mixing has taken place. This carried out means vibratory tissue disintegrator 11 placed container 14 at one end reed 13 vibrated electromagnet 22. After this violently agitated cells disrupted living substance, cause release about hundred ATP molecules per cell. Because pre-mixing, all or most react almost immediately upon from cell, produce burst light which can distinguished individual photons produced stray noise within apparatus.