Technical procedures for the study of organogenesis in vitro in Amblystoma.

摘要: Summary1. A technic is described for rendering surface and subsurface tissues of amphibia wholly sterile use in tissue culture. Advantage taken the nontoxic selective antibiotic action small concentrations sodium sulfadiazine streptomycin.2. medium which supports organogenesis, differentiation growth isolated organ system primordia amphibian embryos has been reported. The principal components this are citrated plasma, salts, agar, glucose nucleate.3. Morphogenetic activity an explanted piece occurs only when epidermis heals so as completely to cover explant.