Development of immunological assays to monitor pulmonary allograft rejection.

摘要: BACKGROUND--At present the diagnosis of pulmonary allograft rejection is made after examination transbronchial biopsy specimens; this method highly invasive. A study was performed to determine whether immunological parameters measured in peripheral blood or bronchoalveolar lavage samples correlate with histological rejection. METHODS--Left unilateral allotransplantation between dogs. The animals were immunosuppressed cyclosporin transplantation but dose drug gradually reduced allow controlled take place. Rejection diagnosed histologically. Four investigated: measurement derived T cell proliferation response limited culture interleukin 2; changes frequency donor reactive cytotoxic lymphocytes; assay level binding IgG antibody recipient plasma; and dependent mediated cells labelling plasma. RESULTS--Assays based on function produced significant results at a time later than severe increased that corresponded closely This did not activate effector mechanism extent. CONCLUSIONS--Measurement specific immunoreactivity can yield data which are indicative These methods make use be obtained by minimally invasive methods. Measurement plasma appears most useful assay. However, each vitro assays used during series experiments less sensitive onset routine examination.