The use of 3-hydroxy-2-naphthoic acid hydrazide and Fast Blue B for the histochemical detection of lipid peroxidation in animal tissues - A microphotometric study
作者： A. Pompella , M. Comporti
DOI: 10.1007/BF00266775
关键词:
摘要: The possibility of detecting lipid peroxidation histochemically by means the 3-hydroxy-2-naphthoic acid/Fast Blue B (NAH-FBB) reaction was evaluated microspectrophotometrically. procedure modified in order to prevent exposure tissue sections solvents. In fresh rat or mouse liver cryostat exposed vitro various prooxidant conditions (NADPH-Fe2+, NADPH-ADP/Fe3+, BrCCl3-NADPH), a close correlation found between intensity NAH-FBB (blue-violet) stain and amount malondialdehyde — taken as biochemical index released incubation medium. Stain intensities obtained with were several fold higher than those obtainable direct Schiff previously used better paralleled time appearance tissue. particular, selective delipidation it observed that is remarkably more efficient protein phospholipid-associated peroxidation-derived carbonyl functions. ability reveal occurring tissues vivo verified animals intoxicated toxins, i.e. haloalkanes bromotrichloromethane carbon tetrachloride, glutathione-depleting agent bromobenzene. livers from haloalkane-treated rats, positivity provided specific absorption spectrum centrolobular regions. bromobenzene-poisoned mice, besides also kidney (tubular epithelium) lung (bronchiolar epithelium). use therefore suggested for discrimination cell types undergoing vivo.
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