Detection of subnanogram quantities of DNA and RNA on native and denaturing polyacrylamide and agarose gels by silver staining
作者： Joseph G. Guillemette , Peter N. Lewis
关键词:
摘要: A silver staining method is described for detecting subnanogram amounts of DNA and RNA on polyacrylamide agarose gels. The key step increased sensitivity linearity an initial soaking the gel in a 0.1% solution cetyltrimethylammonium bromide (CTAB). Agarose-containing gels can be stained after treatment with formaldehyde. methods are comparatively rapid, extremely sensitive (≅ 150 pg DNA) inexpensive (0.4 g AgNo per 25 ml slab volume).
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