Isolation of Human Placental Fibroblasts
作者： Dusko Ilic , Mirhan Kapidzic , Olga Genbacev
DOI: 10.1002/9780470151808.SC01C06S5
关键词:
摘要: The first human embryonic stem cell lines (hESCs) were derived using mouse fibroblasts as feeder cells. In attempts to replace with feeders of origin, irradiated placental successfully used cells for the derivation and propagation hESCs. Here we describe a protocol isolation expansion from villous stroma. We include description architecture provide background stepwise tissue digestion that leads Villous stroma trimester is different term placenta contains mesenchymal, fibroblast-like cells, only few blood vessels, network matrix fibers. isolated single 6- 8-weeks gestation proliferate rapidly retain ability support hESC growth between passage doubling (PD) 8 PD 12. Curr. Protoc. Stem Cell Biol. 5:1C.6.1-1C.6.17. © 2008 by John Wiley & Sons, Inc. Keywords: placenta; fibroblasts; human feeders; human
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