Microbial Leakage of Gutta-percha and Resilon™ Root Canal Filling Material : A Comparative Study Using a new Homogeneous Assay for Sequence Detection
作者： Damiano Pasqualini , Nicola Scotti , Livio Mollo , Elio Berutti , Emma Angelini
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摘要: The sealing ability of gutta-percha/sealer root canal filling was compared to a new thermoplastic synthetic polymer-based obturation material (Resilon TM), using microleakage model and sequence detection assay One Cut Event AmplificatioN (OCEANTM). Eighty-eight extracted human teeth, shaped with K-Files the ProTaper Technique, were randomly assigned four groups (n = 22) obturated in apical 5 mm. Group R Resilon/Epiphany technique; group GP gutta-percha Zinc oxide eugenoe sealer; RCH GPCH received calcium hydroxide intracanal medication before being obturated. Sterilized specimens inoculated Enterococcus faecalis incubated sterile medium for 47 days. DNA from amplified by PCR then identified OCEAN technique. Samples Resilon showed greater number events than other (p 0.036). Calcium medicat...
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