Differential Regulation of Glucose Transport and Transporters by Glucose in Vascular Endothelial and Smooth Muscle Cells

摘要: Hyperglycemia has been implicated in the pathogenesis of both micro- and macrovascular complications diabetes. Little is known, however, about glucose transporters their regulation vascular system. In this study, by was examined cultured BAECs BSMCs, human arterial smooth muscle cells. Both BSMCs transported via facilitated diffusion transport Glucose-transport activity cells inversely reversibly regulated glucose. Exposure HSMCs to high decreased Vmax for 2DG 3-O-MG uptake, whereas Km remained unchanged. The hexose-transport system exhibited lower uptake compared with showed little or no adaptation changes ambient Northern blot analysis demonstrated that GLUT1 mRNA levels were unaffected concentration medium. GLUT2-5 could not be detected analysis. protein, quantified Western analysis, more abundant than approximately 50% when medium elevated from 1.2 22 mM 24 h. alterations level protein correlated observed activity. These observations suggest differential transporter response between endothelial sites autoregulation may involve translational control and/or stability cells.(ABSTRACT TRUNCATED AT 250 WORDS)