Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases

摘要: DNA polymerases catalyze the addition of deoxyribonucleotides onto primers in a template-directed manner. The requirement for template instruction distinguishes these enzymes from other nucleotidyl transferases, such as terminal deoxynucleotidyl transferase, that do not utilize template. An oligonucleotide substrate was used to characterize novel, non-templated nucleotide reaction carried out by variety procaryotic and eucaryotic sources. products reaction, which deoxyribonucleotide added 3' hydroxyl terminus blunt-ended substrate, were analyzed electrophoresis on high resolution, denaturing polyacrylamide gels. polymerase Thermus aquaticus, alpha chick embryo, rat beta, reverse transcriptase avian myeloblastosis virus, I Saccharomyces cerevisiae all blunt-end reaction. required duplex but did require coding information strand. These results demonstrate is an absolute catalysis transfer reactions polymerases.