In vitro studies regarding the feasibility of bovine erythrocyte xenotransfusion

摘要: :  Pigs are considered the most likely source of organs and tissues should barriers to xenotransplantation be overcome. The use animal blood for transfusion, xenotransfusion, would have advantages over from random human donors with respect supply infection control. Large animals such as cows more suitable than pigs donation because easier venous access large volume phlebotomy. Blood 12 Holstein was typed then tested hemagglutination assay (HA), complement mediated lysis (CML), IgM IgG antibody binding, anti-human globulin augmented clinical cross-match osmotic fragility normal serum. Results were compared porcine erythrocytes (pRBC) type O controls (hRBC). frequency ultra-low xenoantigen expressors in a larger herd various breeds using HA CML. Median CML titers one six (no – 64) 26 64), respectively bovine (bRBC). Hemagglutination titer significantly higher pRBC at 170 (one four 1024). lowest group J. Repeated negative bRBC cow that also by seven nine sera representing different groups. However, flow cytometry showed all bound IgG. mean channel fluorescence (MCF) positively correlated titer. corpuscular pRBC, bRBC, hRBC 0.56, 0.48 0.41%, respectively. HA-negative CML-negative 20 35%, herds 49 animals. Bovine RBC elicit variable vitro responses serum but these uniformly much less those seen pRBC. is robust These characteristics including potential ease collection make donor pig.