Long Non-Coding RNA LEF1-AS1 Promotes Migration, Invasion and Metastasis of Colon Cancer Cells Through miR-30-5p/SOX9 Axis.
作者： Ting Sun , Zhexian Liu , Rui Zhang , Siping Ma , Tao Lin
DOI: 10.2147/OTT.S232839
关键词:
摘要: Introduction Aberrant expression of long non-coding RNAs (lncRNAs) has been implicated in the tumorigenesis and progression colon cancer. Lymphoid enhancer-binding factor 1 antisense RNA (LEF1-AS1), a highly conserved newly discovered RNA, reported to be upregulated correlated with poor prognosis cancer, but exact role it remains uncertain. Materials methods In our study, biological functions LEF1-AS1 cancer were analyzed by cell viability assay, colony formation scratch wound healing transwell invasion soft agar luciferase reporter pull down tumor xenograft model Western blot. Results We found that was patients overall survival recurrent-free survival. Besides, enforced HT29 T84 cells promoted migration, invasion, anchorage-independent growth, lung metastasis, while knockdown COLO320 suppressed growth formation. addition, directly interacted inversely miR-30-5p SOX9 downstream target for miR-30-5p. overexpression increased level SOX9, restoration attenuated effects caused Conclusion Our results indicated metastasis partially through miR-30-5p/SOX9 axis. The oncogenic could potential prognostic biomarker
sci-hub.se 参考文章(46)
Bingjian Lü, Jing Xu, Maode Lai, Hao Zhang, Jian Chen, A transcriptome anatomy of human colorectal cancers BMC Cancer. ,vol. 6, pp. 40- 40 ,(2006) , 10.1186/1471-2407-6-40
Ming-Yii Huang, Hung-Chang Chen, I-Ping Yang, Hsiang-Lin Tsai, Tsu-Nai Wang, Suh-Hang Hank Juo, Jaw-Yuan Wang, Tumorigenesis and tumor progression related gene expression profiles in colorectal cancer. Cancer Biomarkers. ,vol. 13, pp. 269- 279 ,(2013) , 10.3233/CBM-130350
Janel L. Kopp, Guido von Figura, Erin Mayes, Fen-Fen Liu, Claire L. Dubois, John P. Morris, Fong Cheng Pan, Haruhiko Akiyama, Christopher V.E. Wright, Kristin Jensen, Matthias Hebrok, Maike Sander, Identification of Sox9-Dependent Acinar-to-Ductal Reprogramming as the Principal Mechanism for Initiation of Pancreatic Ductal Adenocarcinoma Cancer Cell. ,vol. 22, pp. 737- 750 ,(2012) , 10.1016/J.CCR.2012.10.025
C-J Kao, A Martiniez, X-B Shi, J Yang, C P Evans, A Dobi, R W deVere White, H-J Kung, miR-30 as a tumor suppressor connects EGF/Src signal to ERG and EMT. Oncogene. ,vol. 33, pp. 2495- 2503 ,(2014) , 10.1038/ONC.2013.200
Geoffrey Ku, Iain BeeHuat Tan, Thomas Yau, Narikazu Boku, Sudsawat Laohavinij, Ann-Lii Cheng, Yoon-Koo Kang, Gilberto de Lima Lopes, None, Management of colon cancer: resource-stratified guidelines from the Asian Oncology Summit 2012 Lancet Oncology. ,vol. 13, ,(2012) , 10.1016/S1470-2045(12)70424-2
Jian-Jun Zhao, Jianhong Lin, Di Zhu, Xujun Wang, Daniel Brooks, Ming Chen, Zhang-Bo Chu, Kohichi Takada, Bryan Ciccarelli, Samir Admin, Jianguo Tao, Yu-Tzu Tai, Steven Treon, Geraldine Pinkus, Winston Patrick Kuo, Teru Hideshima, Mary Bouxsein, Nikhil Munshi, Kenneth Anderson, Ruben Carrasco, miR-30-5p functions as a tumor suppressor and novel therapeutic tool by targeting the oncogenic Wnt/β-catenin/BCL9 pathway Cancer Research. ,vol. 74, pp. 1801- 1813 ,(2014) , 10.1158/0008-5472.CAN-13-3311-T
Zhongcheng Shi, Chi-I Chiang, Toni-Ann Mistretta, Angela Major, Yuko Mori-Akiyama, SOX9 directly regulates IGFBP-4 in the intestinal epithelium. American Journal of Physiology-gastrointestinal and Liver Physiology. ,vol. 305, ,(2013) , 10.1152/AJPGI.00086.2013
C T Gordon, T Y Tan, S Benko, D FitzPatrick, S Lyonnet, P G Farlie, Long-range regulation at the SOX9 locus in development and disease Journal of Medical Genetics. ,vol. 46, pp. 649- 656 ,(2009) , 10.1136/JMG.2009.068361
Leonardo Salmena, Laura Poliseno, Yvonne Tay, Lev Kats, Pier Paolo Pandolfi, A ceRNA Hypothesis: The Rosetta Stone of a Hidden RNA Language? Cell. ,vol. 146, pp. 353- 358 ,(2011) , 10.1016/J.CELL.2011.07.014
M. M. Center, A. Jemal, R. A. Smith, E. Ward, Worldwide Variations in Colorectal Cancer CA: A Cancer Journal for Clinicians. ,vol. 59, pp. 366- 378 ,(2009) , 10.3322/CAAC.20038