Comparative efficacy of seven hand sanitizers against murine norovirus, feline calicivirus, and GII.4 norovirus.

摘要: Contaminated hands or inanimate surfaces can act as a source of infection during outbreaks human norovirus infection. We evaluated the virucidal efficacy seven hand sanitizers containing various active ingredients, such ethanol, triclosan, and chlorhexidine, compared their effectiveness against feline calicivirus (FCV), murine (MNV), GII.4 fecal extract. also tested 50, 70, 90% ethanol isopropanol. Reduction viral infectivity was measured by plaque assay, number genomic copies determined with TaqMan real-time reverse transcription PCR assay. Based on results quantitative suspension test, only one ethanol-based product (72% pH 2.9) triclosan-based (0.1% 3.0) reduced both MNV FCV (by >2.6 ≥3.4 log units, respectively). Four products were effective either FCV, whereas chlorhexidine ineffective viruses. For these sanitizers, no correlation found between decline RNA. Ethanol isopropanol concentrations ≥70% ≥2.6 50 70% ≥2.2 units after exposure for 5 min. The susceptibility to low relative high alcohols suggest that surrogate viruses should be considered in vitro testing sanitizers.