The length of the bound fatty acid influences the dynamics of the acyl carrier protein and the stability of the thioester bond.

摘要: Acyl carrier proteins involved in fatty acid biosynthesis have been shown to exhibit a high degree of conformational flexibility, that they are able sequester intermediates between 4 and 18 carbons length. This flexibility has observed X-ray NMR structures acyl attached different acids. studies comparing decanoyl-ACP stearoyl-ACP indicated ACP exhibits more dynamic motions when bound longer We used complementary chemical methods as an approach improving our understanding the effect length on dynamics protein. A assay accessibility thioester solvent revealed positive correlation chain rate hydrolysis. Surprisingly, this linear was biphasic, with accelerated hydrolysis for acids than 15 carbons. To further understand associated acceleration, we collected 15N...