Hydrophilic-interaction planar chromatography in ultra-sensitive determination of α-aminocephalosporin antibiotics. Application to analysis of cefalexin in goat milk samples using modified QuEChERS extraction technique.

摘要: Abstract A highly sensitive, selective and precise HPTLC method coupled with fluorescence detection was developed validated for the determination of α-aminocephalosporin antibiotics; namely cefalexin, cefadroxil cefradine in their standard solutions. The applicability methodology demonstrated via analysis cefalexin goat milk samples. Full optimization derivatization reaction carried out regard to solutions studied compounds or after extraction separation performed on precoated silica gel plates 60 F254 using acetonitrile: water a ratio 85:15 (v/v) as mobile phase. retention behavior formed derivatives discussed detail. It found that hydrophilic interaction mode is main mechanism governing derivatives. In addition, an experimental design approach conducted chromatographic conditions. Modified QuEChERS applied efficient technique from both spiked real Optimization achieve highest recovery results indicate this provides good (83–116%) Limit (LOD) be 0.023, 0.005, 0.023 ng band−1 cefradine, respectively 0.165 ng According achieved LOD values, sensitivity quasi-equivalent other methods based expensive techniques such HPLC-UV HPLC-MS it sufficient determine below its MRL Moreover, successfully pharmacokinetic study single intramuscular injection 10 mg kg−1 per body weight.