Strand displacement amplification-coupled dynamic light scattering method to detect urinary telomerase for non-invasive detection of bladder cancer
作者： Jing Wang , Ji Zhang , Tingting Li , Ruidi Shen , Gongke Li
DOI: 10.1016/J.BIOS.2019.02.014
关键词:
摘要: Despite huge successes achieved by strand displacement amplification (SDA) and gold nanoparticles (AuNPs) in biomolecules sensing, the strategy of combination SDA AuNPs-based dynamic light scattering (DLS) for a biomolecule sensing is unexplored. Here we developed non-invasive, SDA-based DLS method diagnosis bladder cancer detecting telomerase activity human urine. In presence telomerase, substrate (TS) primer was elongated with repeating sequences (TTAGGG)n, resulting product triggers between hairpin deoxyribonucleic acid (DNA) Primer. The can be recognized oligonucleotide-modified AuNPs probes, measurable aggregation. assay displayed detection limit 3 MCF-7 cells signal-to-noise ratio range 5-1000 cells. simple, reliable has been successfully applied urine good accuracy, selectivity reproducibility. Moreover, only samples from patients induced significant change average hydrodynamic diameter, indicating practical applicability non-invasive cancer.
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