Identification of Sp1 as a Transcription Activator to Regulate Fibroblast Growth Factor 21 Gene Expression.

摘要: Fibroblast growth factor 21 (FGF21) is a metabolic hormone with multiple beneficial effects on lipid and glucose homeostasis. Previous study demonstrated that FGF21 might be one of the Sp1 target genes. However, transcriptional role in adipose tissue liver has not been reported. In this study, we found proximal promoter mouse located between -63 -20 containing two putative Sp1-binding sites. mammalian transcription involved regulation many genes during physiological pathological processes. Our showed overexpression or suppressing expression resulted increased reduced activity, respectively. Mutation analysis site -46 -38 plays primary FGF21. Electrophoretic mobility shift assay chromatin immunoprecipitation indicated specifically bound to region. Furthermore, binding activity was significantly tissues HFD-induced obese DEN-treated mouse. Thus, our results demonstrate positively regulates basal contributes obesity-induced upregulation hepatic hepatocarcinogenesis.