Sister chromatid exchange frequency in cultured isolated porcine urinary bladder epithelial cells (PUBEC) treated with ochratoxin A and alpha
作者： W. Föllmann , I. E. Hillebrand , E. E. Creppy , H. M. Bolt
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摘要: The mycotoxin ochratoxin A (OTA) and its metabolite alpha (OT-alpha) were investigated, to examine their potency induce sister chromatid exchages (SCE) in cultured poricine urinary baldder epithedial cells (PUBEC) (primary cluture). Serum-free PUBEC incubated for 5 h with either OTA or OT-alpha, respectively, subsequently cutured the presence of 5-bromo-2-deoxyuridine (BrdU). After two cell cycles, mitosis was inhibited by colchicine derivative Colcemid, fixed chromosomes prepared SCE analysis. For OTA, a dose-dependent increase frequency measured concentrations between 100 pM nM OTA. At 100nM increased about 41%, compared base level (7.27 SCEs per chromosome set, solvent control). Higher cytotoxic. OT-alpha also frequency, but at higher concentrations. concentration 10μM an 55% detected. showed no cytotoxic effect. There results indicate that is genotoxic this vitro system, which represents bladder epithelium, target organ vivo. It could be shown said non-toxic, assay
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