Interference of deferasirox with assays for serum iron and serum unsaturated iron binding capacity during iron chelating therapy

摘要: Abstract Background Deferasirox (DFX) is an oral iron chelator that used worldwide for the treatment of overload. Although serum ferritin level usually measured as a marker efficacy DFX, we sometimes experienced unexplainable changes in other markers iron. We hypothesized photometric assays (sFe) and unsaturated binding capacity (UIBC) might be affected by DFX. Methods Measurement sFe UIBC was performed using 4 different assay systems. The samples were prepared adding 0–300 μM DFX to pooled human or 15 randomized samples. In some experiments, DFX-iron complex (DFX-Fe) mixing ammonium citrate solution solution. Results influenced DFX-Fe, while iron-free showed no effect on value sFe; DFX-Fe sFe, undistinguishable from transferrin-bound On hand, measurement itself; have been bound reagent assay, leading increase values. Conclusions must careful observing these during chelation therapy with