DNA damage induced by genotoxicants in zebrafish (Danio rerio) embryos after contact exposure to freeze-dried sediment and sediment extracts from Laguna Lake (The Philippines) as measured by the comet assay

摘要: Abstract Laguna Lake, Philippines, is utilised as a resource for drinking and irrigation water major source of animal protein more than 11 million residents. However, it also serves huge sink anthropogenic pollutants from variety direct or indirect discharges. Given their well-known properties to accumulate organic pollutants, Lake sediments have been investigated by use newly developed sediment-contact assay determine the bioavailable fraction total genotoxic hazard potential. Freshly fertilized zebrafish eggs (2 h after fertilization) were allowed develop while exposed different concentrations freeze-dried (exposure compounds) well extracts (complete potential including bioaccumulation) prepared collected at five sites within lake. Following 96 h exposure, single-cell suspensions macerated larvae analyzed DNA strand-breaks comet assay. Genotoxicity could be identified in both solid-phase exposure scenarios sediment extracts. Only South Bay (SB) site did not show significant burden relative controls tests In contrast, Northwest displayed pronounced genotoxicity embryos scenarios. Perylene copper causes response. To compare results (maximum induction coefficients) with an established protocol, rainbow trout liver cells (RTL-W1) same The findings correlated (Spearman correlation r  = 0.90), proving good reliability primary cells. conclusion, present study demonstrates that may pose threat benthic organisms human health via drinking-water fish consumption.