Stomach lysozyme gene of the langur monkey: Tests for convergence and positive selection

摘要: Genomic blotting and enzymatic amplification show that the genome of langur monkey (like other primates) contains only a single gene for lysozymec, in contrast to another group foregut fermenters, ruminants, which have multigene family encoding this protein. Therefore, stomach lysozyme has probably evolved recently (i.e., within period evolution) from conventional primate lysozyme. The sequences cDNAs lysozymes three Old World monkeys were determined. Identification promoter comparison human gene, is expressed conventionally macrophages, both genes use same promoter. This suggests difference expression patterns due change(s) enhancer or silencer regulatory elements. With cDNA hypothesis converged amino acid sequence upon ruminants tested. Consistent with convergence hypothesis, those sites specify acids mature are shared uniquely ruminant genes. None silent at third positions codons noncoding regions support link between ruminants. Statistical analysis based on rules out possibility horizontal transfer lineages supports close relationship monkeys.