Protein Engineering of Surface Loops: Preliminary X-Ray Analysis of the Chy155–165rhi Mutant
作者： Raj R. V. Dhanaraj , Jim E. Pitts , Phil Nugent , Poonsook Orprayoon , Jon B. Cooper
DOI: 10.1007/978-1-4615-1871-6_11
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摘要: The protein engineering programme at Birkbeck seeks to develop generic methods based on a design cycle involving biochemical preparation and characterization, determination comparative analysis of three-dimensional structures, rule-based design, site-directed mutagenesis expression the mutants. Knowledge tertiary structure proteins use molecular modelling techniques provide powerful approach for novel biomolecules with specifically engineered properties. aspartic proteinase family have been studied in detail by X-ray suitable data base development principles1–6. This endopeptidases belonging wide range biological species varying substrate specificities. members are considerable commercial importance since enzymes such as chymosin Mucor pusillus pepsin exploited food industry cheese soya processing, while renins, cathepsins retroviral proteinases prime targets pharmaceutical industries drug design.
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