Construction method of tissue engineered tissue based on artificial cell

作者: Tang Xiaolin , He Peng , Lin Qin , Liu Yuangang , Chen Aizheng

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摘要: The invention provides a construction method of tissue engineered based on an artificial cell. comprises the following steps of: floating cell suspension in filtered and sterilized sodium alginate solution, dropping into calcium chloride solution through liquid generation device to form bead; placing bead membrane material contained coating surface microcapsule, wherein is cleaned by using normal saline; then, neutralizing superfluous positive charges microcapsule; liquefying microcapsule citrate cell; next, curing forming mixture aquogel finally complex; complex under static or oscillatory microgravity condition culture construct tissue. By method, requirement traditional support for high porosity connectivity rate can be reduced; simple beneficial realization large-scale production in-vitro construction.

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