Glycine Betaine as a Direct Substrate for Methanogens (Methanococcoides spp.)

摘要: Nine marine methanogenic Methanococcoides strains, including the type strains of methylutens, M. burtonii, and alaskense, were tested for utilization N-methylated glycines. Three (NM1, PM2, MKM1) used glycine betaine (N,N,N-trimethylglycine) as a substrate methanogenesis, partially demethylating it to N,N-dimethylglycine, whereas none N,N-dimethylglycine or sarcosine (N-methylglycine). Growth rates growth yields per mole with (3.96 g [dry weight] mol) similar those trimethylamine (4.11 mol). However, is only demethylated, yield methyl group was significantly higher than trimethylamine. If are provided together, demethylated dimethyl- methylamine limited utilization. After depleted, dimethylamine consumed rapidly, before methylamine. Glycine extends range substrates that can be directly utilized by some methanogens, allowing them gain energy from without need syntrophic partners.