Biaxial mechanics and inter-lamellar shearing of stem-cell seeded electrospun angle-ply laminates for annulus fibrosus tissue engineering.

摘要: The annulus fibrosus (AF) of the intervertebral disk plays a critical role in vertebral load transmission that is heavily dependent on microscale structure and composition tissue. With degeneration, both are compromised, resulting loss AF mechanical function. Numerous tissue engineering strategies have addressed issue but few focused recapitulation microstructure One approach allows for generation engineered with appropriate (+/−)30° lamellar use aligned electrospun scaffolds seeded mesenchymal stem cells (MSCs) assembled into angle-ply laminates (APL). Previous work indicates opposing orientation necessary development near native uniaxial tensile properties. However, most loads applied biaxially, as subjected to multi-axial constrained by its attachments bodies. Thus, objective this study was evaluate biaxial response bilayers, determine importance under loading regime. Opposing which replicate structure, showed significantly higher modulus testing directions compared parallel reached ∼60% Associated increase properties, less shear, stretch fiber direction, observed. These results provide additional insight structure–function relationships, well new benchmarks functional constructs. © 2013 Orthopaedic Research Society. Published Wiley Periodicals, Inc. J Orthop Res 31: 864–870,