RNA editing in gymnosperms and its impact on the evolution of the mitochondrial coxI gene.

作者: Meng-Zhu Lu , Alfred E. Szmidt , Xiao-Ru Wang

DOI: 10.1023/A:1005972513322

关键词:

摘要: Sequence analysis of the mitochondrial coxI gene in eight gymnosperm species revealed a high rate non-synonymous nucleotide substitutions with strong (98%) predominance C-T substitutions. Further corresponding cDNA sequences showed that all changes genomic DNA were eliminated by RNA editing resulting nearly identical mRNA (amino acid) among species. Pronounced variation number and location edited sites was found Most had relatively large (from 25 to 34). However, no sequence Gingko biloba or Larix sibirica. The composition investigated fragment suggests G. L. sibirica originated from transcripts reverse transcription followed insertion into nuclear genome back genome. Our results also demonstrate where there are sites, can accelerate divergence

参考文章(26)
Alejandro Araya, Dominique Begu, Simon Litvak, RNA editing in plants Physiologia Plantarum. ,vol. 91, pp. 543- 550 ,(1994) , 10.1111/J.1399-3054.1994.TB02986.X
T. Wakasugi, T. Hirose, M. Horihata, T. Tsudzuki, H. Kossel, M. Sugiura, Creation of a novel protein-coding region at the RNA level in black pine chloroplasts: the pattern of RNA editing in the gymnosperm chloroplast is different from that in angiosperms Proceedings of the National Academy of Sciences of the United States of America. ,vol. 93, pp. 8766- 8770 ,(1996) , 10.1073/PNAS.93.16.8766
S. Kwok, D.E. Kellogg, N. McKinney, D. Spasic, L. Goda, C. Levenson, J.J. Sninsky, Effects of primer-template mismatches on the polymerase chain reaction: Human immunodeficiency virus type 1 model studies Nucleic Acids Research. ,vol. 18, pp. 999- 1005 ,(1990) , 10.1093/NAR/18.4.999
Wen-Hsiung Li, Unbiased estimation of the rates of synonymous and nonsynonymous substitution Journal of Molecular Evolution. ,vol. 36, pp. 96- 99 ,(1993) , 10.1007/BF02407308
Kenneth H Wolfe, Wen-Hsiung Li, Paul M Sharp, Rates of nucleotide substitution vary greatly among plant mitochondrial, chloroplast, and nuclear DNAs. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 84, pp. 9054- 9058 ,(1987) , 10.1073/PNAS.84.24.9054
JosepM. Comeron, A METHOD FOR ESTIMATING THE NUMBERS OF SYNONYMOUS AND NONSYNONYMOUS SUBSTITUTIONS PER SITE Journal of Molecular Evolution. ,vol. 41, pp. 1152- 1159 ,(1995) , 10.1007/BF00173196