Cloning and characterization of the Pseudomonas aeruginosa lasR gene, a transcriptional activator of elastase expression.

摘要: We report the discovery of lasR gene, which positively regulates elastase expression in Pseudomonas aeruginosa PAO1. The gene was cloned by its ability to restore a positive phenotype strain PA103, possesses structural (lasB) but fails synthesize enzyme. Nucleotide sequence analysis revealed an open reading frame 716 nucleotides encoding protein approximately 27 kDa. A labeled LasR kDa detected Escherichia coli using T7 RNA polymerase system. chromosomal deletion mutant constructed PAO1 replacement. This (PAO-R1) is devoid elastolytic activity and antigen. deduced amino acid 27% homologous activator LuxR Vibrio fischeri suspected 28K-UvrC E. coli. Northern (RNA) total cellular from PAO1, PAO-R1, PAO-R1 containing on multicopy plasmid (pMG1.7) that functional required for transcription (lasB). Images