Structure and in vitro anticancer activity of sulfated O-polysaccharide from marine bacterium Poseidonocella pacifica KMM 9010 T
作者： Maxim S. Kokoulin , Alexandra S. Kuzmich , Anatoly I. Kalinovsky , Eugene S. Rubtsov , Lyudmila A. Romanenko
DOI: 10.1016/J.CARBPOL.2017.09.052
关键词:
摘要: Abstract We presented the structure of sulfated polysaccharide moiety and anticancer activity in vitro О-deacylated lipopolysaccharide (DPS) isolated from marine bacterium Poseidonocella pacifica KMM 9010T. The O-polysaccharide was investigated by chemical methods along with 1H 13C NMR spectroscopy. built up disaccharide repeating units consisted d -rhamnose ( -Rhaр) 3-deoxy- -manno-oct-2-ulosonic acid (Kdop): →7)-β-Kdoр4Ac5S-(2→3)-β- -Rhaр2S-(1→. demonstrated that DPS P. 9010T non-toxic for normal mouse epidermal cells (JB6 Cl41 cell line) inhibited colony formation human colorectal carcinoma HT-29, breast adenocarcinoma MCF-7 melanoma SK-MEL-5 a dose-dependent manner.
sci-hub.se 参考文章(15)
John A. Barltrop, Terence C. Owen, Ann H. Cory, Joseph G. Cory, 5-(3-carboxymethoxyphenyl)-2-(4,5-dimethylthiazolyl)-3-(4-sulfophenyl)tetrazolium, inner salt (MTS) and related analogs of 3-(4,5-dimethylthiazolyl)-2,5-diphenyltetrazolium bromide (MTT) reducing to purple water-soluble formazans As cell-viability indicators Bioorganic & Medicinal Chemistry Letters. ,vol. 1, pp. 611- 614 ,(1991) , 10.1016/S0960-894X(01)81162-8
Lyudmila A. Romanenko, Naoto Tanaka, Vasily I. Svetashev, Nataliya I. Kalinovskaya, Poseidonocella pacifica gen. nov., sp. nov. and Poseidonocella sedimentorum sp. nov., novel alphaproteobacteria from the shallow sandy sediments of the Sea of Japan Archives of Microbiology. ,vol. 194, pp. 113- 121 ,(2012) , 10.1007/S00203-011-0736-3
Aizhen Zong, Hongzhi Cao, Fengshan Wang, Anticancer polysaccharides from natural resources: A review of recent research Carbohydrate Polymers. ,vol. 90, pp. 1395- 1410 ,(2012) , 10.1016/J.CARBPOL.2012.07.026
Sergey Fedorov, Svetlana Ermakova, Tatyana Zvyagintseva, Valentin Stonik, Anticancer and cancer preventive properties of marine polysaccharides: some results and prospects. Marine Drugs. ,vol. 11, pp. 4876- 4901 ,(2013) , 10.3390/MD11124876
Martin Lenter, Barbara Jann, Klaus Jann, Structure of the K16 antigen from Escherichia coli O7:K16:H-, a Kdo-containing capsular polysaccharide. Carbohydrate Research. ,vol. 197, pp. 197- 204 ,(1990) , 10.1016/0008-6215(90)84142-H
Karin Leontein, Bengt Lindberg, Jörgen Lōnngren, Assignment of absolute configuration of sugars by g.l.c. of their acetylated glycosides formed from chiral alcohols Carbohydrate Research. ,vol. 62, pp. 359- 362 ,(1978) , 10.1016/S0008-6215(00)80882-4
Per-Erik Jansson, Lennart Kenne, Göran Widmalm, Computer-assisted structural analysis of polysaccharides with an extended version of casper using 1H- and 13C-n.m.r. data Carbohydrate Research. ,vol. 188, pp. 169- 191 ,(1989) , 10.1016/0008-6215(89)84069-8
Evgeny L. Nazarenko, Russell J. Crawford, Elena P. Ivanova, The Structural Diversity of Carbohydrate Antigens of Selected Gram-Negative Marine Bacteria Marine Drugs. ,vol. 9, pp. 1914- 1954 ,(2011) , 10.3390/MD9101914
U. K. LAEMMLI, Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 Nature. ,vol. 227, pp. 680- 685 ,(1970) , 10.1038/227680A0
W F Vann, T Soderstrom, W Egan, F P Tsui, R Schneerson, I Orskov, F Orskov, Serological, chemical, and structural analyses of the Escherichia coli cross-reactive capsular polysaccharides K13, K20, and K23. Infection and Immunity. ,vol. 39, pp. 623- 629 ,(1983) , 10.1128/IAI.39.2.623-629.1983