Our panel of experts highlight the most important research articles across the spectrum of topics relevant to the field of regenerative medicine

摘要: Enzymatically crosslinked microporous hydrogel scaffolds that form in situ promote dermal healing Evaluation of: Griffin DR, Weaver WM, Scumpia PO, DiCarlo D, Segura T. Accelerated wound by injectable gel assembled from annealed building blocks. Nat. Mater. 14, 737–744 (2015). Tunable respond to environmental factors could enable enhanced tissue integration and healing. To achieve this goal, scaffold designs have incorporated biomimetic functionality, including thrombin-activated factor XIII (FXIIIa) crosslinking [1], matrix metalloproteinase responsiveness [2] mechanical matching [3]. Scaffolds can alter their properties response stimuli are desired changes stiffness, shape, release degradation lead improved regeneration. In study, et al. describe a bottom-up strategy fabricating accelerate [4]. They use microfluidic approach microparticle (microgel) blocks establish interconnected network structures upon FXIIIa-mediated crosslinking. These particles (MAPs) incorporate reactive multiarmed poly(ethylene)glycol-vinyl sulfone, the commonly used RGD cell adhesion peptide K Q noncanonical transglutaminase substrates with cysteine-terminated metalloproteinase-sensitive peptides allow for cell-controlled material degradation. FXIIIa addition be injected into sites solidification shape-conformation. MAPs mechanically robust tuned storage moduli 10 1000 Pa, which fall within stiffness range of soft tissues. vitro studies demonstrate MAP 3D cellular formation proliferation survival after 6 days. vivo SKH1-HR Balb/c epidermal models 24 h, significant closure as compared nonporous chemically matched controls 5 days or physically control up 7 following injection scaffolds, keratin-5, keratin-14 CD49f staining revealed re-epithelialization, PECAM-1, NG2 PDGFR-β indicated promoted complex vascular development. were also able sustain normal hair follicle when tissue. The less CD11b infiltration days, suggests cause lower immune response. Taken together, accelerated utilizing natural enzymatic mechanisms mimicking favorable facilitate proliferation. study may benefited evaluating closure, regeneration Our panel experts highlight most important research articles across spectrum topics relevant field regenerative medicine