Experimental Dissection of Protein-Protein Interactions in Solution

摘要: Publisher Summary Protein–protein interactions play crucial roles in a wide variety of processes, which define cell structure and function. This chapter describes the emerging tools strategies for determining kinetics thermodynamics protein–protein interactions. These biophysical methods can be used to dominant as core studying structural functional molecular properties In general, these require relatively small amounts (milligrams) protein material are universally applicable study proteins. Although capable providing molecular-level details interactions, increase resolution level is accompanied by an increased need validate interpretation data. The discusses synergistic benefits using multiple independent methods.