Droplet-interface-bilayer assays in microfluidic passive networks.

摘要: Basic biophysical studies and pharmacological processes can be investigated by mimicking the intracellular extracellular environments across an artificial cell membrane construct. The ability to reproduce in vitro simplified scenarios found live membranes automated manner has great potential for a variety of synthetic biology compound screening applications. Here, we present fully integrated microfluidic system production lipid bilayers based on miniaturisation droplet-interface-bilayer (DIB) techniques. platform uses design that enables controlled positioning storage phospholipid-stabilized water-in-oil droplets, leading successfully scalable formation arrays DIBs mimic processes. To ensure robustness operation, have how concentration, immiscible phase flow velocities device geometrical parameters affect performance. Finally, produced proof-of-concept data showing diffusive transport molecules ions on-chip studied quantified using fluorescence-based assays.