Multiplex PCR Analysis for Rapid Detection of Klebsiella pneumoniae Carbapenem-Resistant (Sequence Type 258 [ST258] and ST11) and Hypervirulent (ST23, ST65, ST86, and ST375) Strains.

摘要: Carbapenem-resistant and hypervirulent Klebsiella pneumoniae strains have emerged recently. These are both multidrug resistant may also be highly transmissible able to cause severe infections in the hospital community. Clinical public health needs require a rapid comprehensive molecular detection assay identify track spread of these provide timely infection control information. Here, we develop multiplex PCR capable distinguishing K. carbapenem-resistant isolates sequence type 258 (ST258) ST11, ST23, ST65/ST375, ST86 clones, as well capsular types K1, K2, K locus 47 (KL47), KL64, virulence genes rmpA, rmpA2, iutA, iroN. The demonstrated 100% concordance with 118 previously genotyped revealed different populations two collections China United States. results showed that still rare States, whereas China, ∼50% carry rmpA/rmpA2 iutA genes, which largely associated epidemic ST11 strains. Similarly, high prevalence was found carbapenem-susceptible Chinese hospitals, but primarily belong ST86, distinct from Taken together, our this can useful tool for surveillance