High Prevalence of Plasmid-Mediated Quinolone Resistance and IncQ Plasmids Carrying qnrS2 Gene in Bacteria from Rivers near Hospitals and Aquaculture in China

摘要: Effluents from hospital and aquaculture are considered important sources of quinolone resistance. However, little information is available on the impact this effluent nearby rivers. In study, 188 ciprofloxacin-resistant bacterial isolates obtained rivers near hospitals were screened for plasmid-mediated resistance (PMQR) genes. Species identification, antibiotic susceptibility testing, PMQR gene transferability assessment conducted PMQR-positive bacteria. Representative qnrS2-encoding plasmids subsequently sequenced using a primer-walking approach. total, 44 (23.4%) positive qnr genes (16 qnrB2, 3 qnrS1, 25 qnrS2) 32 (17.0%) aac(6')-Ib-cr. Other not detected. The qnrB2 aac(6')-Ib-cr had higher prevalence in samples than samples, significantly associated with Enterobacteriaceae (p < 0.05). contrast, qnrS2 was site-related, but Aeromonas spp. All resistant to three or more classes antibiotics. Eleven qnrS2-harboring spp., including novel conjugative plasmid pHP18, selected sequencing. These small size (6,388-16,197 bp) belonged IncQ IncU family, being part mobile insertion cassette. Taken together, our findings suggest that possible source dissemination, demonstrate ubiquity aquatic environments. Finally, served as vectors help IncQ-type plasmids.