Isolation of a Streptococcus pyogenes gene locus that directs hyaluronan biosynthesis in acapsular mutants and in heterologous bacteria.

摘要: A contiguous 3-kilobase pair region of DNA was isolated from Group Streptococcus pyogenes (GAS) that can direct hyaluronic acid (HA) capsule biosynthesis in acapsular mutants as well heterologous bacteria. The identified by transposon 916 insertional mutagenesis and subcloned into a plasmid shuttle vector. Mutant GAS or Enterococcus faecalis containing this plasmid, but not vector alone, displayed mucoid phenotype on agar plates, possessed seen light microscopy, produced HA quantities comparable with wild-type GAS. polysaccharide shown to be authentic based its recognition specific proteoglycan degradation Streptomyces hyaluronate lyase. Escherichia coli the complementing also at only 10% level made above cells. E. minicell analysis showed two proteins, 42 45 kDa, are expressed functional insert. Deletion insert minicells revealed 42-kDa protein is essential for production. This first demonstration reconstitution vivo.