Tumor Cells

摘要: This chapter discusses existing methods for detection and characterization of disseminated tumor cells (DTCs) in blood bone marrow patients with epithelial tumors, clinical implications, suitability DTCs as surrogate markers therapeutic success. enter the circulation via bloodstream or lymphatic vessels migrate to new sites where they extravasate into surrounding tissue. There, may either exist single develop micrometastases. In both cases, must initiate maintain growth until angiogenesis forms vascularized metastases. The characteristics can be very different from that primary tissue originate from. Some tumors metastatic cell clones are reported independently diverge such a way neoplasm disappears only metatases remain detectable. Whereas polymerase chain reaction (PCR), flow cytometry fluorescence situ hybridization (FISH) have become standard techniques hematopoietic malignancies, standardization solid is still process. Extraction mRNA amplification reverse transcriptase (RT-PCR) analysis preferred method practice. Prognostic relevance cancers briefly discussed. Though numerous studies tried link presence prognostic significance, findings contradictory hard compare. results listed table chapter.