Betacellulin and activin A coordinately convert amylase-secreting pancreatic AR42J cells into insulin-secreting cells.

摘要: Rat pancreatic AR42J cells possess exocrine and neuroendocrine properties. Activin A induces morphological changes converts them into neuron-like cells. In activin-treated cells, mRNA for polypeptide (PP) but not that either insulin or glucagon was detected by reverse transcription-PCR. About 25% of the were stained anti-PP antibody. When incubated with betacellulin, a small portion positively antiinsulin antibodies. The effect betacellulin dose dependent, being maximal at 2 nM. Approximately 4% became positive this concentration, mRNAs PP detected. combination activin A, approximately 10% positive. Morphologically, insulin-positive composed two types cells: round-shaped Immunoreactive found in latter type insulin, PP, glucose transporter 2, glucokinase, glucagon, Depolarizing concentration potassium, tolbutamide, carbachol, glucagon-like peptide-1 stimulated release immunoreactive insulin. These results indicate convert amylase-secreting secreting provide model system to study formation endocrine