STATIC AND KINETIC SITE-SPECIFIC PROTEIN-DNA PHOTOCROSSLINKING: ANALYSIS OF BACTERIAL TRANSCRIPTION INITIATION COMPLEXES
作者： Nikolai Naryshkin , Sergei Druzhinin , Andrei Revyakin , Younggyu Kim , Vladimir Mekler
DOI: 10.1007/978-1-60327-015-1_25
关键词:
摘要: Static site-specific protein-DNA photocrosslinking permits identification of interactions within multiprotein-DNA complexes. Kinetic photocrosslinking--involving rapid-quench-flow mixing and pulsed-laser irradiation--permits elucidation pathways kinetics formation We present detailed protocols for application static kinetic to bacterial transcription initiation
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