Design and performance evaluation of ionic liquid-based microwave-assisted simultaneous extraction of kaempferol and quercetin from Chinese medicinal plants

摘要: An ionic liquid-based microwave-assisted extraction (IL-MAE) technique has been successfully developed for the simultaneous effective of quercetin and kaempferol from Toona sinensis Rosa chinensis which are Chinese medicinal plants. Twenty different types liquids (ILs) with cations anions have synthesized investigated. Several significant operating parameters IL-MAE were optimized by a series single factor experiments orthogonal design L9 (34) experiments, respectively. Compared maceration (IL-ME), heat (IL-HE) ultrasound-assisted (IL-UAE), approach was demonstrated as optimal highest efficiency shortest time. Reversed phase high performance liquid chromatography (RP-HPLC) ultraviolet-detection (UV-DAD) employed analysis quercetin. The results indicated that amounts extracted using [Bmim]Br [Omim]Br 176.5 mg g−1 RSD 1.2% 134.3 0.63%. And 16.12 0.24% 9.070 0.15%. analyzing linear between concentrations 32.10 L−1 to 233.3 in 25.70 160.5 regression coefficients (R) 0.9998 0.9999, kaempferol, 0.73 23.20 0.19 15.00 0.9993 0.9996, recoveries methods 96.76–104.1% 98.40–103.3% chinensis, 95.75–106.3% 98.55–102.9% RSDs lower than 3.0%. LODs 1.87–2.26 μg mL−1. microstructures chemical structures these two researched samples before after procedures also