Semiquantitative cytochemistry in evaluation of apoptotic capacity in bronchoalveolar lavage of smokers and patients with non-small-cell lung cancer

摘要: Apoptotic capacity of pulmonary tissue to produce or remove apoptotic cells by alveolar macrophages (ALMs) was investigated in three groups: healthy volunteers, smokers and patients with non-small-cell lung cancer (NSCLC). Differential cell counting bronchoalveolar lavage (BAL) specimens revealed significantly higher percentages neutrophils eosinophils decreased percentage BAL NSCLC comparison nonsmokers smokers. Proportion lymphocytes than These changes the profile may reflect immunology malignancies. were lower AMs increased nonsmokers. This result might be understood as a consequence changed architecture situ, influenced smoking. detection cytocentrifuge preparations suspensions evaluated TUNEL method. Subsequent steps, adsorption, internalization digestion (AMs) estimated semiquantitative cytochemical scoring indexing method correlated percent free cells. Significant increase control (289.55 ′ 50.77) that non-smokers (218.29 56.24) could stimulated inside AMs; (150.30 40.61; p<0.05), is relation reduced phagocytosis remnants, which either cause oncogenic process.