CD133 Expression Is Not Synonymous to Immunoreactivity for AC133 and Fluctuates throughout the Cell Cycle in Glioma Stem-Like Cells

摘要: A transmembrane protein CD133 has been implicated as a marker of stem-like glioma cells and predictor for therapeutic response in malignant brain tumours. expression is commonly evaluated by using antibodies specific the AC133 epitope located one extracellular domains membrane-bound CD133. There conflicting evidence regarding significance identifying predicting degree malignancy cells. The reasons discrepant results between different studies addressing role CD133/AC133 gliomas are unclear. possible source controversies about widespread assumption that patterns reflect linearly those protein. Consequently, readouts from assessments often interpreted terms purpose this study to determine whether what extent do obtained with anti-AC133 antibody correspond level expressed Our reveals first time on surface poorly immunoreactive AC133. Furthermore, we provide occupancy fluctuates during cell cycle. offer new explanation numerous inconsistencies biological clinical human call caution interpreting lack or presence