Heterologous prime-boost vaccination with a non-replicative vaccinia recombinant vector expressing LACK confers protection against canine visceral leishmaniasis with a predominant Th1-specific immune response.

摘要: Leishmaniasis caused by Leishmania infantum is a severe endemic disease in the Mediterranean basin, being domestic dogs main reservoir of that plays key role transmission to humans. Studies on vaccines against canine leishmaniasis, aimed modify T cell repertoire, have advanced recent years. LACK vaccination assays, using protein or DNA vectors, show protection cutaneous L. major infections redirecting early IL-4 responses protective Th1 response. The aim this study was define effectiveness and type immune response visceral leishmaniasis model two poxvirus vectors (Western reserve strain, WR modified vaccinia virus Ankara, MVA) expressing prime/boost protocols. results obtained showed dog priming with DNA-LACK followed booster MVA-LACK rVV-LACK triggered response, leading leishmaniasis. This correlated absence symptoms, lower Leishmania-specific antibodies, higher degree activation Leishmania-target organs synthesis cytokines. In addition, we found boosted non-replicative less VL symptoms activation, providing evidences for clear advantage as vector