A site-specific, conservative recombination system carried by bacteriophage P1. Mapping the recombinase gene cin and the cross-over sites cix for the inversion of the C segment.

摘要: Abstract The bacteriophage P1 genome carries an invertible C segment consisting of 3-kb unique sequences flanked by 0.6-kb inverted repeats. With insertion and deletion mutants derivatives the site-specific recombinase gene cin for inversion) has been mapped adjacent to cix sites (for inversion cross-over) have located at outside ends Inversion functions as a biological switch controls expression gene(s) responsible phage infectivity carried on segment. The product can promote recombination between 'quasi- ' site plasmid pBR322 DNA. junctions formed resulting co-integrate also serve sites. This observation implies potential evolutionary process bring genes under control acting DNA inversion.