Role of substituents in cyclodextrin derivatives for enantioselective gas chromatographic separation of chiral terpenoids in the essential oils of Mentha spicata

摘要: Enantioselective GC-FID and enantioselective GC-MS have been utilized under temperature gradient mode with differently substituted heptakis- octakis-cyclodextrins to achieve the resolution of chiral terpenoids in essential oil indigenously grown cultivars Mentha spicata. Modified cyclodextrins were derivatized GC column for separation terpenoids. A 2,3-diethyl-6-tert-butyldimethylsilyl-β-cyclodextrin doped into 14% cyanopropylphenyl/86%dimethylpolysiloxane (TBDE-β-CD) showed good enantioselectivity all studied compounds excluding carvone. Carvone enantiomers well resolved 2,3-diacetoxy-6-tert-butyldimethylsilyl-β-cyclodextrin (TBDA-β-CD) enantioseparation (Es) 1.006. TBDE-β-CD provides maximum enantiomeric β-pinene (Es 1.038), sabinene 1.051), limonene 1.045), isomenthone 1.029) α-terpineol 1.014). Furthermore, enantiomer elution order reversal was observed sabinene, menthone, terpinen-4-ol menthol while changing from β- γ-cyclodextrin phase. exhibits by substituents i.e., methyl acetyl at 2- & 3- position cyclodextrin derivative. Chiral constituents such as (+)-isomenthone, (-)-menthone, (1R,2S,5R)-(-)-menthol (4S)-(+)-piperitone exist a single >99% excess. Existence (R)-(+)-limonene (S)-(+)-carvone has proven first time M. spicata oils can be used marker Indian origin.