Effect of intra-tumoral magnetic nanoparticle hyperthermia and viral nanoparticle immunogenicity on primary and metastatic cancer.

摘要: Although there is long association of medical hyperthermia and immune stimulation, the relative lack a quantifiable reproducible effect has limited utility advancement this relationship in preclinical/clinical cancer non-cancer settings. Recent cancer-based findings (immune checkpoint modulators etc.) including improved mechanistic understanding biological tools now make it possible to modify exploit system benefit conventional treatments such as radiation hyperthermia. Based on prior experience our research group including; heat therapy, magnetic nanoparticle (mNP) hyperthermia, biology, immunology Cowpea Mosaic Virus that been engineered over express antigenic proteins without RNA or DNA (eCPMV/VLP). This was designed determine if how intra-tumoral delivery mNP VLP can work together improve local systemic tumor treatment efficacy. Using C3H mouse/MTG-B mammary adenocarcinoma cell model C57-B6 mouse/B-16-F10 melanoma model, data suggests appropriate combination (e.g. 43°C/30-60 minutes) (100 μg/200 mm3 tumor) not only result significant primary regression but creation reaction potential retard secondary growth (abscopal effect) resist rechallenge. Molecular from these experiments suggest based damage signals Heat Shock Protein (HSP) 70/90, calreticulin, MTA1 CD47 are targets be exploited enhance treatment.