Doppler Ultrasound Imaging Detects Changes in Tumor Perfusion during Antivascular Therapy Associated with Vascular Anatomic Alterations

摘要: Noninvasive monitoring of antiangiogenic therapy was performed by serial power Doppler ultrasound imaging murine tumors treated with recombinant interleukin 12, the results which were correlated assessments tumor vascularity microscopy. Growth established K1735 tumors, but not IFN-γ-unresponsive K1735.N23 variants, suppressed treatment. Serial during treatment revealed a progressive change from diffuse perfusion pattern to more punctate distribution. Quantitative analysis images that color-weighted fractional average, representing overall perfusion, consistently decreased in these primarily because decrease cross-sectional area carrying blood flow. In contrast, parameters increased nonresponsive Confocal microscopy thick sections reduction density and arborization vessels labeled vivo fluorochrome-conjugated lectin effective Immunohistological examination thin confirmed preferential loss small successful therapy. Similar changes vascular anatomy also observed 12 two other responsive types. These indicate is sensitive, noninvasive method for reporting functional consequences therapy-induced anatomical can be used serially monitor efficacy antivascular clinical trials.