The use of decellularised animal tissue to study disseminating cancer cells

摘要: Since the establishment of cell culture, common practice has been to grow adherent cells in 2D monolayers. Although behave completely differently when grown under these artificial conditions, ease culturing meant that this still prevails, and adopting conditions more closely reflect natural microenvironment met with substantial inertia. The alternative, animal models mimic human physiology, are less accessible, strictly regulated require licences expensive facilities. transition from 3D is gathering momentum, there a clear need for alternative methods resemble vivo conditions. Here, we show decellularised organs gleaned discarded carcasses ideal biomimetic scaffolds support secondary tumour initiation vitro Further, describe how decellularise tissue perform basic histochemistry immunofluorescence procedures matrix detection. Cancer behaviour on followed by way an example. Because integration into traditional work flow easy inexpensive, hope article will encourage other researchers adopt approach.