Regulation of bcl-2 gene expression in lymphoid cell lines containing normal #18 or t(14;18) chromosomes.

摘要: The bcl-2 (B cell lymphoma/leukemia-2) gene at band 18q21 is involved in t(14;18) chromosomal translocations most follicular lymphomas and occasional other human B malignancies, where it becomes juxtaposed to the transcriptionally active immunoglobulin (Ig) locus 14q32. Regulation of expression was investigated neoplastic lymphoid lines containing normal #18 chromosomes or a translocation with regard steady-state mRNA levels, RNA stability, transcription rates, DNA methylation. High levels mRNA, proportionally high rates (measured isolated nuclei), were found translocations. half-life (approximately 2-3 hr) similar all examined, including t(14;18)-containing lymphoma line, which has translocated rearranged that produces bcl-2/Ig fusion transcripts. However, presence cycloheximide (inhibitor protein synthesis), some mRNAs produced by these cells prolonged, indicating circumstances stability may contribute deregulated expression. Despite stabilizing mRNAs, overall effect treating reduction accumulated through inhibition transcription. These latter data provide indirect evidence short-lived transacting factor(s) regulate without translocation. No clear correlation discovered between methylation