Expression Profile of Six RNA-Binding Proteins in Pulmonary Sarcoidosis.

摘要: Background Sarcoidosis is characterised by up-regulation of cytokines and chemokine ligands/receptors proteolytic enzymes. This pro-inflammatory profile regulated post-transcriptionally RNA-binding proteins (RBPs). We investigated in vivo expression six RBPs (AUF1, HuR, NCL, TIA, TIAR, PCBP2) two inhibitors enzymes (RECK, PTEN) pulmonary sarcoidosis compared it to the four control groups healthy individuals patients with other respiratory diseases: chronic obstructive disease (COPD), asthma idiopathic interstitial pneumonias (IIPs). Methods RT-PCR was used quantify mRNAs bronchoalveolar (BA) cells obtained from 50 patients, 23 controls, 30 COPD, 19 asthmatic IIPs patients. Flow cytometry assess intracellular protein AUF1 HuR peripheral blood T lymphocytes (PBTLs) 9 6 controls. Results Taking stringent conditions for multiple comparisons into consideration, we consistently observed primary analysis including all regardless smoking status as well subsequent sub-analysis limited never smokers that BA mRNA (p<0.001), TIA NCL (p<0.01) RECK (p<0.05) decreased controls. also both diseases (COPD asthma; p<0.001) but not IIPs. There were several positive correlations between RBP cells. Also CD3+, CD4+ CD8+ PBTLs displayed lower mean fluorescence intensity (p≤0.02) (p≤0.03) than PBTLs. Conclusion mRNA expressions three NCL) their potential target encoding additionally down-regulated our individuals. Its significance, e.g. stability factors, should be further explored sarcoidosis.