Surfactant metabolism and anti-oxidative capacity in hyperoxic neonatal rat lungs: effects of keratinocyte growth factor on gene expression in vivo

摘要: Development of preterm infant lungs is frequently impaired resulting in bronchopulmoary dysplasia (BPD). BPD results from interruption physiologic anabolic intrauterine conditions, the inflammatory basis and therapeutic consequences premature delivery, including increased oxygen supply for air breathing. The latter requires surfactant, produced by alveolar type II (AT II) cells to lower surface tension at pulmonary air:liquid interface. Its main components are specific phosphatidylcholine (PC) species dipalmitoyl-PC, anionic phospholipids surfactant proteins. Local antioxidative enzymes essential cope with pro-inflammatory side effects normal pressures. However, respiratory insufficiency supply. To injurious hyperoxia epithelia, recombinant human keratinocyte growth factor (rhKGF) was proposed as a stimulating, non-catabolic epithelial-protective therapeutic. aim present study examine qualification rhKGF improve expression parameters lung maturity newborn rats under hyperoxic conditions (85 % O2 7 days). In response proliferating cell nuclear antigen mRNA, feature stimulated proliferation, elevated. Similarly, expressions ATP-binding cassette protein A3 gene, differentiation marker AT peroxiredoxin 6, thioredoxin reductase, three genes involved radical protection were increased. Furthermore, mRNA levels acyl-coA:lysophosphatidylcholine acyltransferase 1, catalyzing dipalmitoyl-PC synthesis acyl remodeling, adipose triglyceride lipase, considered responsible fatty acid PC synthesis, These results, together considerable body other confirmative evidence, suggest that should be developed into option treat infants risk development.