A novel integrin α5β1 antagonistic peptide, A5-1, screened by Protein Chip system as a potent angiogenesis inhibitor
作者： Eung-Yoon Kim , Ji Young Bang , Soo-Ik Chang , In-Cheol Kang
DOI: 10.1016/J.BBRC.2008.10.166
关键词:
摘要: Integrin alpha5beta1 immobilized on a ProteoChip was used to screen new antagonistic peptides from multiple hexapeptide sub-libraries of the positional scanning synthetic peptide combinatorial library (PS-SPCL). The integrin alpha5beta1-Fibronectin interaction demonstrated chip. A novel ligand, A5-1 (VILVLF), with high affinity identified libraries this chip-based screening method basis competitive inhibition assay. inhibits integrin-fibronectin in dose-dependent manner (IC(50); 1.56+/-0.28 microM. In addition, it human umbilical vein endothelial cell proliferation, migration, adhesion, tubular network formation, and bFGF-induced neovascularization chick chorioallantoic membrane. These results suggest that will be potent inhibitor neovascularization.
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